Which item is NOT required to be in a vehicle at all times?

The correct answer is that a first aid kit is not required to be in a vehicle at all times. While having a first aid kit in your vehicle is certainly beneficial and can be useful in emergencies, it is not a legal requirement for all drivers.

On the other hand, the other items mentioned are essential. A spare tire is important for addressing flat tires and ensuring mobility in case of emergencies. Current vehicle registration is necessary for legal driving and proves that the vehicle is roadworthy and legally compliant. A driver's license is mandatory as it certifies that the driver is legally qualified to operate the vehicle. Without each of these items, you could face penalties or legal issues, whereas the absence of a first aid kit does not carry the same legal implications.